The lab states fixation will induce the dyes to leak from your cells. I feel the idea is always that fixation may well hurt mobile membranes. I did look for a paper which describes fixation employing methanol or acetic acid, following staining by using a DCFDA by-product. DCFDA is the https://2-fdckhcl24680.like-blogs.com/29134583/the-smart-trick-of-2-fdck-hcl-that-no-one-is-discussing